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2锛夊噵浜℃娓�(c猫)

鈶� 娴佸紡鍑嬩骸妾㈡脯(c猫)锛氱窗(x矛)鑳炶啘绲勫垎纾烽叝閰扮挡姘ㄩ吀锛�PS锛夊湪姝ｅ父鎯呮硜涓嬩綅浜庣窗(x矛)鑳炶啘鍏�(n猫i)鍋�(c猫)锛岀暥(d膩ng)绱�(x矛)鑳炲姝绘垨鑰呭噵浜℃檪(sh铆)锛�PS閬风Щ鑷崇窗(x矛)鑳炶啘澶栧伌(c猫)銆傜７鑴傜祼(ji茅)鍚堣泲鐧�V锛�Annexin V锛夋槸涓€绋垼渚濊炒鎬х殑纾疯剛绲�(ji茅)鍚堣泲鐧�锛屽畠鑸�PS鍏锋湁楂樺害鐨勭祼(ji茅)鍚堝姏銆傜啋鍏夌礌FITC妯�(bi膩o)瑷樼殑Annexin V鍙互浣滅偤鎺㈤嚌妾㈡脯(c猫)鏆撮湶鍦ㄧ窗(x矛)鑳炶啘澶栧伌(c猫)鐨勭７閰叞绲叉皑閰�锛屾寚绀哄姝绘垨鍑嬩骸鐨勭窗(x矛)鑳�銆�FITC-Annexin V绲�(ji茅)鍚堝埌绱�(x矛)鑳炲悗锛屽湪钘�(l谩n)鑹插厜鐨勬縺鐧�(f膩)涓�锛岀櫦(f膩)鍑虹稜鑹茬啋鍏夈€傜鍖栦笝鍟讹紙Propidium iodide锛�PI锛夋槸涓€绋牳閰告煋鏂�锛屽畠涓嶈兘閫忛亷(gu貌)瀹屾暣绱�(x矛)鑳炶啘锛屼絾灏�(du矛)鍑嬩骸鏅氭湡绱�(x矛)鑳炲拰姝荤窗(x矛)鑳炵殑鐮存悕绱�(x矛)鑳炶啘鑳藉绌块€�锛屽苟浣跨窗(x矛)鑳炴牳绱呮煋锛岃€岀窗(x矛)鑳炶啘淇濇寔瀹屽ソ鐨勬棭鏈熷噵浜＄窗(x矛)鑳炲墖涓嶆渻(hu矛)鏈夌磪鑹茬啋鍏夌敘(ch菐n)鐢�銆傝伅(li谩n)鍚堟噳(y墨ng)鐢�Annexin V-FITC鍜�PI灏�(du矛)鍩归(y菐ng)楂旂郴涓殑绱�(x矛)鑳為€�(j矛n)琛屾煋鑹�锛�Annexin V锛�-锛�/PI锛�-锛変唬琛ㄥ仴搴锋椿绱�(x矛)鑳�锛�Annexin V锛�+锛�/PI锛�-锛変唬琛ㄦ棭鏈熷噵浜＄窗(x矛)鑳�锛�Annexin V锛�+锛�/PI锛�+锛変唬琛ㄦ櫄鏈熷噵浜＄窗(x矛)鑳炲拰澹炴绱�(x矛)鑳炪€�

鈶� Hechst鏌撹壊锛氱窗(x矛)鑳炵櫦(f膩)鐢熷噵浜℃檪(sh铆)锛屾煋鑹茶唱(zh矛)鏈�(hu矛)鍥虹府銆傛墍浠�Hoechst 33258鏌撹壊鍚庯紝鍦ㄧ啋鍏夐’寰彙涓嬭瀵�锛屾甯哥窗(x矛)鑳炵殑绱�(x矛)鑳炴牳鍛堟甯哥殑钘�(l谩n)鑹诧紝鑰屽噵浜＄窗(x矛)鑳炵殑绱�(x矛)鑳炴牳鏈�(hu矛)鍛堣嚧瀵嗘績鏌�锛屾垨鍛堢濉婄媭鑷村瘑婵冩煋锛岄鑹叉湁浜涚櫦(f膩)鐧�銆�

鈶� Tunle妾㈡脯(c猫)锛氱窗(x矛)鑳炲湪鐧�(f膩)鐢熷噵浜℃檪(sh铆)锛屾渻(hu矛)婵€娲讳竴浜�DNA鍏�(n猫i)鍒囬叾锛岄€欎簺鍏�(n猫i)鍒囬叾鏈�(hu矛)鍒囨柗鏍稿皬楂旈枔鐨勫熀鍥犵祫DNA銆傜窗(x矛)鑳炲噵浜℃檪(sh铆)鎶芥彁DNA閫�(j矛n)琛岄浕娉虫娓�(c猫)锛屽彲浠ョ櫦(f膩)鐝�(xi脿n)180-200 bp鐨�DNA ladder?锛涱媰铓狹DNA鏂疯鏅�(sh铆)锛屾毚闇茬殑3鈥�-OH鍙互鍦ㄦ湯绔劔姘ф牳鑻烽吀杞�(zhu菐n)绉婚叾锛�Terminal Deoxynucleotidyl Transferase锛�TdT锛夌殑鍌寲涓嬪姞涓婄稜鑹茬啋鍏夋帰閲濈啋鍏夌礌锛�FITC锛夋(bi膩o)瑷樼殑dUTP锛�luorescein-dUTP锛夛紝寰炶€屽彲浠ラ€氶亷(gu貌)鐔掑厜椤井閺℃垨娴佸紡绱�(x矛)鑳炲剙閫�(j矛n)琛屾娓�(c猫)銆�

3锛夐伔绉诲拰渚佃ゲ

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